Characteristics of the type II collagen (CII)-specific T cell line. CII-specific T cell line QDHT during passage 5 was used in this study. An aliquot of the cell line used for transfer was tested for proliferation (a) and interferon-γ secretion (b) after stimulation with different peptides. Mouse and rat CII (mK and rK, respectively), hydroxylated rat CII (rHyK), denatured rat CII (dCII) and mouse and rat galactosylated CII (mGalHyK and rGalHyK, respectively) peptides were used. Rat CII was heat denatured at 50°C for 20 min. Results are representative of several experiments performed with this cell line.
Nandakumar et al. Arthritis Res Ther 2004 6:R544 doi:10.1186/ar1217