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Open Access Research article

Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction

Mamoru Yoshida1*, Shigaku Sai1, Keishi Marumo1, Takaaki Tanaka1, Naoki Itano2, Koji Kimata2 and Katsuyuki Fujii1

Author Affiliations

1 Department of Orthopaedic Surgery, The Jikei University School of Medicine, Tokyo, Japan

2 Institute for Molecular Science of Medicine, Aichi Medical University, Aichi, Japan

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Arthritis Res Ther 2004, 6:R514-R520  doi:10.1186/ar1223

Published: 22 September 2004

Abstract

Hyaluronan is a major molecule in joint fluid and plays a crucial role in joint motion and the maintenance of joint homeostasis. The concentration and average molecular weight of hyaluronan in the joint fluids are reduced in osteoarthritis and rheumatoid arthritis. To elucidate the underlying mechanism, we analyzed the message expression of three isoforms of hyaluronan synthase and hyaluronidase from knee synovium, using real-time reverse transcriptase polymerase chain reaction. Synovia were obtained from 17 patients with osteoarthritis, 14 patients with rheumatoid arthritis, and 20 healthy control donors. The message expression of hyaluronan synthase-1 and -2 in the synovium of both types of arthritis was significantly less than in the control synovium, whereas that of hyaluronidase-2 in the synovium of both arthritides was significantly greater than in the control synovium. The decreased expression of the messages for hyaluronan synthase-1 and -2 and/or the increased expression of the message for hyaluronidase-2 may be reflected in the reduced concentration and decreased average molecular weight of hyaluronan in the joint fluids of patients with osteoarthritis and rheumatoid arthritis.

Keywords:
arthritis; hyaluronan; hyaluronan synthase; hyaluronidase; synovium