Table 2

Cytokine release from draining lymph node cells of collagen-induced arthritis mice


Cytokine release from DLN cells


Treatment
IFN-γ (ng/ml): 48 hour CII
IFN-γ (ng/ml): 48 hour ConA
IL-4 (pg/ml): 48 hour CII
IL-4 (pg/ml): 48 hour ConA

GTRTEmpty + doxycycline
0.41 ± 0.03
4.2 ± 1.1
11.9 ± 3.2
33.2 ± 8.8
GTRTT – doxycycline
0.66 ± 0.15
2.4 ± 0.8
6.4 ± 1.5
24.4 ± 6.7
GTRTT + doxycycline
0.49 ± 0.04
2.8 ± 0.9
9.0 ± 1.6
21.5 ± 2.4

Results are presented from at least six animals in each group. Cytokine release from draining lymph node (DLN) cells was induced by 48 hours of stimulation with CII or ConA and was measured by ELISA. CII, collagen type II.

Gould et al. Arthritis Res Ther 2004 6:R103   doi:10.1186/ar1036