Characterization of cultured human prosthesis loosening fibroblasts (PLFs) by flow cytometry. (a) When compared with isotype control staining, analysis for the macrophage lineage marker CD68 showed no surface expression (<0.1%). (b) Also, no expression of the leucocyte common antigen CD45 was found on the cells (<0.1%). (c) However, fluorescent-activated cell sorter analysis with the fibroblast marker D7-Fib revealed the fibroblast nature of the cells (>99%). (d) In addition, more than 99% of the PLFs stained positive for the fibroblast marker AS02.
Pap et al. Arthritis Res Ther 2003 5:R163-R173 doi:10.1186/ar752