Table 2

Deregulated genes in the arthritic SF as revealed by differential display RT-PCR

Clone no.

RN

N

Deregulationa

RT-PCR

Gene ID

Accession no.


6

+

+

> 1

NADH dehydrogenase S2

M22756

7

+

+

1.3

(+)

FIN 13

U42383

8

+

+

> 1

Aldose Reductase

U93231.1

9,17,19

+

+

2.1/4.8/8.6

Ribosomal protein L3

U89417

10

+

+

2.7

NADH dehydrogenase S4

AF100726

13

+

4

SPARC/osteonectin

X04017

14,39,71

+

+

-5

MHC-1b H2-T23 (Qa1-like)

U12822

15,35

+

+

2.2

EST~GMP reductase

AA240130

16

+

+

- 70

mt. cytochrome b

AF159396.1

18

+

+

> 1

ATP-specific succinyl-CoA synthetase β

AF058955

20,25

+

+

1.6/5

Hsp70

M34561

21,58

+

+

1.8/6.3

HnRNP D-like / JKTBP

AB017020

22,24

+

+

2.26

+

ZO-2

U75916

23

+

+

10

+

HSPC194

AF151028

27

+

+

4

Unknown

28

+

+

-14.7

Smoothelin large isoform L2

AF064236.1

29

+

?

< -1

+

EST~RNA binding protein

L17076/S72641

29b

+

?

< -1

EST

AI013881

31

(+)

+

> 1

+

Unknown

32,33

+

+

3

+

Unknown

34

+

(+)

> 1

Unknown

36

+

?

> 1

Unknown

37

+

?

> 1

Hypothalamus protein HT001

AF113539

38

+

?

< -1

Ran-GTP binding protein

Y08890

Karyopherin b3

NM002271.1

41

+

(+)

> 1

Huntingtin int. prot. 1 family

AF049613

HSPC136

AF161485

42

+

+

> 1

Unknown

43

(+)

+

< -1

Pyruvate kinase (PK3)-M2 subunit

NM011099

44

+

> 1

HSPC030

AF170920

45,46,47

+

+

2.1

Ribosomal protein L7a

X15013.1

48

+

+

36

(+)

Homologue to eIF6/integrin b4 int. prot.

AF081140

49

+

+

8.8

+

HSPC249 (from CD34+ stem cells)

AF151083

50

+

> 1

Unknown

51

+

?

> 1

Unknown

52

+

+

72?

(+)

EAP330 of ELL

NM007241

53

+

+

> 1

Ferritin heavy chain

NM010239

54

(+)

+

- 7.9

Ly-6E.1 alloantigen

X04653

TAP (T cells activating pr)

M59713.1

55

(+)

?

< -1

E124 (etoposide induced/+p53)

U41751

56

+

?

> 1

EST

AA960119

57

(+)

?

> 1

mt DNA polymerase γ

U53584

60

+

?

> 1

EST

AA963457

61

(+)

+

2.3

Karyopherin a4 (importin a3)?

NM002268

62

+

+

3.5

+

LIM-protein?

AF037208

65

+

+

22

MEKK4?

NM011948

66

+

?

> 1

Human mRNA expr. in thyroid gland

D83198

66b

+

?

> 1

Human cDNA FLJ20657 fis

AK000664

67

+

?

> 1

Unknown

68

+

?

> 1

Unknown

69

+

(+)

> 1

Unknown

70

+

?

< -1

MHC class II

AF110520


aFold of up/down-regulation, as calculated from Northern blots after normalization against glyceraldehyde-3-phosphate dehydrogenase. N, Northern; RN, reverse Northern; RT-PCR, reverse transcriptase polymerase chain reaction.

Aidinis et al. Arthritis Res Ther 2003 5:R140-R157   doi:10.1186/ar749

Open Data