Table 2 |
||||||
|
Deregulated genes in the arthritic SF as revealed by differential display RT-PCR |
||||||
|
Clone no. |
RN |
N |
Deregulationa |
RT-PCR |
Gene ID |
Accession no. |
|
|
||||||
|
6 |
+ |
+ |
> 1 |
NADH dehydrogenase S2 |
M22756 |
|
|
7 |
+ |
+ |
1.3 |
(+) |
FIN 13 |
U42383 |
|
8 |
+ |
+ |
> 1 |
Aldose Reductase |
U93231.1 |
|
|
9,17,19 |
+ |
+ |
2.1/4.8/8.6 |
Ribosomal protein L3 |
U89417 |
|
|
10 |
+ |
+ |
2.7 |
NADH dehydrogenase S4 |
AF100726 |
|
|
13 |
+ |
4 |
SPARC/osteonectin |
X04017 |
||
|
14,39,71 |
+ |
+ |
-5 |
MHC-1b H2-T23 (Qa1-like) |
U12822 |
|
|
15,35 |
+ |
+ |
2.2 |
EST~GMP reductase |
AA240130 |
|
|
16 |
+ |
+ |
- 70 |
mt. cytochrome b |
AF159396.1 |
|
|
18 |
+ |
+ |
> 1 |
ATP-specific succinyl-CoA synthetase β |
AF058955 |
|
|
20,25 |
+ |
+ |
1.6/5 |
Hsp70 |
M34561 |
|
|
21,58 |
+ |
+ |
1.8/6.3 |
HnRNP D-like / JKTBP |
AB017020 |
|
|
22,24 |
+ |
+ |
2.26 |
+ |
ZO-2 |
U75916 |
|
23 |
+ |
+ |
10 |
+ |
HSPC194 |
AF151028 |
|
27 |
+ |
+ |
4 |
Unknown |
||
|
28 |
+ |
+ |
-14.7 |
Smoothelin large isoform L2 |
AF064236.1 |
|
|
29 |
+ |
? |
< -1 |
+ |
EST~RNA binding protein |
L17076/S72641 |
|
29b |
+ |
? |
< -1 |
EST |
AI013881 |
|
|
31 |
(+) |
+ |
> 1 |
+ |
Unknown |
|
|
32,33 |
+ |
+ |
3 |
+ |
Unknown |
|
|
34 |
+ |
(+) |
> 1 |
Unknown |
||
|
36 |
+ |
? |
> 1 |
Unknown |
||
|
37 |
+ |
? |
> 1 |
Hypothalamus protein HT001 |
AF113539 |
|
|
38 |
+ |
? |
< -1 |
Ran-GTP binding protein |
Y08890 |
|
|
Karyopherin b3 |
NM002271.1 |
|||||
|
41 |
+ |
(+) |
> 1 |
Huntingtin int. prot. 1 family |
AF049613 |
|
|
HSPC136 |
AF161485 |
|||||
|
42 |
+ |
+ |
> 1 |
Unknown |
||
|
43 |
(+) |
+ |
< -1 |
Pyruvate kinase (PK3)-M2 subunit |
NM011099 |
|
|
44 |
+ |
> 1 |
HSPC030 |
AF170920 |
||
|
45,46,47 |
+ |
+ |
2.1 |
Ribosomal protein L7a |
X15013.1 |
|
|
48 |
+ |
+ |
36 |
(+) |
Homologue to eIF6/integrin b4 int. prot. |
AF081140 |
|
49 |
+ |
+ |
8.8 |
+ |
HSPC249 (from CD34+ stem cells) |
AF151083 |
|
50 |
+ |
> 1 |
Unknown |
|||
|
51 |
+ |
? |
> 1 |
Unknown |
||
|
52 |
+ |
+ |
72? |
(+) |
EAP330 of ELL |
NM007241 |
|
53 |
+ |
+ |
> 1 |
Ferritin heavy chain |
NM010239 |
|
|
54 |
(+) |
+ |
- 7.9 |
Ly-6E.1 alloantigen |
X04653 |
|
|
TAP (T cells activating pr) |
M59713.1 |
|||||
|
55 |
(+) |
? |
< -1 |
E124 (etoposide induced/+p53) |
U41751 |
|
|
56 |
+ |
? |
> 1 |
EST |
AA960119 |
|
|
57 |
(+) |
? |
> 1 |
mt DNA polymerase γ |
U53584 |
|
|
60 |
+ |
? |
> 1 |
EST |
AA963457 |
|
|
61 |
(+) |
+ |
2.3 |
Karyopherin a4 (importin a3)? |
NM002268 |
|
|
62 |
+ |
+ |
3.5 |
+ |
LIM-protein? |
AF037208 |
|
65 |
+ |
+ |
22 |
MEKK4? |
NM011948 |
|
|
66 |
+ |
? |
> 1 |
Human mRNA expr. in thyroid gland |
D83198 |
|
|
66b |
+ |
? |
> 1 |
Human cDNA FLJ20657 fis |
AK000664 |
|
|
67 |
+ |
? |
> 1 |
Unknown |
||
|
68 |
+ |
? |
> 1 |
Unknown |
||
|
69 |
+ |
(+) |
> 1 |
Unknown |
||
|
70 |
+ |
? |
< -1 |
MHC class II |
AF110520 |
|
|
|
||||||
|
aFold of up/down-regulation, as calculated from Northern blots after normalization against glyceraldehyde-3-phosphate dehydrogenase. N, Northern; RN, reverse Northern; RT-PCR, reverse transcriptase polymerase chain reaction. |
||||||
|
Aidinis et al. Arthritis Res Ther 2003 5:R140 doi:10.1186/ar749 |
||||||
