(a) Representative differential display RT-PCR of the arthritic SF (hTNF/TAg) clone B2 versus the normal (wt/TAg) SF clone F6. I and II are duplicate experiments; b is a duplicate reaction of a, starting with a 1:5 dilution of RNA/cDNA sample. Representative (b) reverse Northern blot, (c) Northern blot, and (d) RT-PCR respectively, as described in Materials and methods. hTNF = human tumor necrosis factor; RT-PCR – reverse transcriptase polymerase chain reaction; SF = synovial fibroblast; TAg = large tumor antigen; wt = wild-type.
Aidinis et al. Arthritis Res Ther 2003 5:R140-R157 doi:10.1186/ar749