Expression of human TNF by SF clones. Anti-hTNF enzyme-linked immunosorbent assay from cell-culture supernatants was carried out as described in Materials and methods. Values are normalized for hTNF production per 1 × 106 cells/ml over a 24-hour period. Mean averages of triplicates with t-test P values less than 0.01. 'Recovered' refers to SFs derived from the diseased ankle of hTNF/TAg SF B2 injected mice (hTNF) or the nondiseased ankle of wt/TAg SF F6 injected mice (wt). hTNF production was assayed after 20 days/4 passages in culture. hTNF = human tumor necrosis factor; SF = synovial fibroblast; TNF = tumor necrosis factor; tsTAg = temperature-sensitive large tumor antigen.
Aidinis et al. Arthritis Res Ther 2003 5:R140-R157 doi:10.1186/ar749