Effects of IL-18 on osteoclast formation. Mouse spleen cells and osteoblasts from wild-type mice (WT), IFN-γ receptor type II-knockout mice (IFNγR KO) or granulocyte–macrophage colony-stimulating factor-knockout mice (GM-CSF KO) were cocultured with 1-α,25(OH)2D3 and prostaglandin E2 (PGE2) in the presence or absence of IL-18. In some cocultures, T-cell-depleted spleen cells and osteoblasts were cocultured in the presence and absence of WT T cells or GM-CSF KO T cells. In each coculture, numbers of tartrate-resistant acid phosphatase-positive osteoclasts formed were scored.
Udagawa et al. Arthritis Res 2002 4:281 doi:10.1186/ar431