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Open Access Research article

Fragmentation of Golgi complex and Golgi autoantigens during apoptosis and necrosis

Kazuhisa Nozawa1, Carlos A Casiano2, John C Hamel1, Christine Molinaro2, Marvin J Fritzler3 and Edward KL Chan1*

Author Affiliations

1 Department of Molecular and Experimental Medicine, WM Keck Autoimmune Disease Center, The Scripps Research Institute, La Jolla, California, USA

2 Department of Medicine and Department of Biochemistry and Microbiology, Center for Molecular Biology and Gene Therapy, Loma Linda University School of Medicine, Loma Linda, California, USA

3 Department of Medicine and Department of Biochemistry and Molecular Biology, University of Calgary, Calgary, Alberta, Canada

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Arthritis Res 2002, 4:R3  doi:10.1186/ar422

Published: 11 June 2002

Abstract

Anti-Golgi complex autoantibodies are found primarily in patients with Sjögren's syndrome and systemic lupus erythematosus, although they are not restricted to these diseases. Several Golgi autoantigens have been identified that represent a small family of proteins. Common features of all Golgi autoantigens appear to be their distinct structural organization of multiple α-helical coiled-coil rods in the central domains flanked by non-coiled-coil N-termini and C-termini, and their localization to the cytoplasmic face of Golgi cisternae. Many autoantigens in systemic autoimmune diseases have distinct cleavage products in apoptosis or necrosis and this has raised the possibility that cell death may play a role in the generation of potentially immunostimulatory forms of autoantigens. In the present study, we examined changes in the Golgi complex and associated autoantigens during apoptosis and necrosis. Immunofluorescence analysis showed that the Golgi complex was altered and developed distinctive characteristics during apoptosis and necrosis. In addition, immunoblotting analysis showed the generation of antigenic fragments of each Golgi autoantigen, suggesting that they may play a role in sustaining autoantibody production. Further studies are needed to determine whether the differences observed in the Golgi complex during apoptosis or necrosis may account for the production of anti-Golgi complex autoantibodies.

Keywords:
anti-Golgi complex antibody; autoantibody; autoimmunity; cell death