A section through the growth plate of a fetal mouse metatarsal, 17 days post fertilization. Blue staining indicates endogenous alkaline phosphatase activity in the hypertrophic chondrocytes, diaphyseal perichondrium and bone collar, and primary spongiosa. Safranin O staining of chondromucin in the cartilage matrix is red. The regions populated by each stage of the chondrocyte lineage are shown.
Shum and Nuckolls Arthritis Res 2002 4:94-106 doi:10.1186/ar396