Table 3

Effects of anti-TWEAK and anti-TNF monoclonal antibodies on the secretion of IL-8, PGE2 and MMP-1 by synoviocytes stimulated by hTWEAK or TNF

Stimulus
None
BCB10 (10 μg/ml)
BEB3 (10 μg/ml)
Anti-TNF (10 μg/ml)



IL-8 secretion (ng/ml)

None
55.2 ± 3.1
38 ± 1.2
117.6 ± 18
49 ± 14
hTWEAK
146.4 ± 17
73 ± 19
277.2 ± 40
113.8 ± 7.3
TNF
232.6 ± 26
245 ± 33
nd
44.7 ± 4.8


MMP-1 secretion (ng/ml)

None
4248 ± 276
3848 ± 371
5383 ± 274
3482 ± 584
hTWEAK
6033 ± 773
4381 ± 22
7923 ± 1698
4878 ± 100
TNF
6197 ± 210
7846 ± 713
nd
3505 ± 101


PGE2 secretion (ng/ml)

None
50 ± 12
61 ± 6
81 ± 13
47 ± 13
HTWEAK
94 ± 7
87 ± 26
161 ± 30
78 ± 3
TNF
231 ± 49
471 ± 79
nd
53 ± 5


RANTES secretion (ng/ml)

None
0.12
0.23
0.23
0.05
hTWEAK
30.78
0.26
20.12
26.01
TNF
2.19
1.17
nd
0.05


IP-10 secretion (pg/ml)

None
274
386 ± 17
349
117 ± 84
hTWEAK
5095 ± 766
330 ± 140
3158
3735 ± 60
TNF
1710 ± 300
1620 ± 20
nd
105

hTWEAK (TNF-like weak inducer of apoptosis) concentration was 10 ng/ml; TNF (tumour necrosis factor) concentration was 1 ng/ml. Values for IL-8, matrix metalloproteinase-1 (MMP-1), prostaglandin E2 (PGE-2) and interferon-γ-inducible protein-10 (IP-10) are shown as mean ± SD; similar results were obtained in 3 independent experiments. Single analyses for RANTES (regulated on activation, normal T expressed and secreted) were done because the amount of available medium was limited. nd, not done.

Chicheportiche et al. Arthritis Res 2002 4:126   doi:10.1186/ar388