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Effects of anti-TWEAK and anti-TNF monoclonal antibodies on the secretion of IL-8, PGE2 and MMP-1 by dermal fibroblasts stimulated by hTWEAK or TNF |
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| Stimulus |
None |
BCB10 (10 μg/ml) |
BEB3 (10 μg/ml) |
Anti-TNF (10 μg/ml) |
|
|
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| IL-8 secretion (ng/ml) |
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| None |
4.9 ± 0.9 |
5.8 ± 0.6 |
5.9 ± 0.9 |
4.6 ± 0.3 |
| hTWEAK (10 ng/ml) |
19.8 ± 2.8 |
6.0 ± 1.2 |
44.6 ± 6.1 |
18.8 ± 2.6 |
| TNF(1 ng/ml) |
74.3 ± 13 |
109.3 ± 11 |
nd |
4.5 ± 0.3 |
| MMP-1 secretion (ng/ml) |
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| None |
392 ± 17 |
494 ± 55 |
523 ± 68 |
362 ± 32 |
| hTWEAK (10 ng/ml) |
789 ± 35 |
588 ± 8 |
1287 ± 69 |
827 ± 21 |
| TNF(1 ng/ml) |
2084 ± 154 |
2863 ± 141 |
nd |
356 ± 37 |
| PGE2 secretion (ng/ml) |
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| None |
33 ± 2 |
24 ± 2 |
90 ± 6 |
44 ± 14 |
| hTWEAK(50 ng/ml) |
47 ± 4 |
25 ± 2 |
103 ± 36 |
52 ± 10 |
| TNF(1 ng/ml) |
190 ± 55 |
212 ± 75 |
nd |
31 ± 5 |
| RANTES secretion (ng/ml) |
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| None |
0.73 |
0.47 |
0.72 |
0.83 |
| hTWEAK(10 ng/ml) |
17.22 |
1.06 |
47.99 |
24.96 |
| TNF(1 ng/ml) |
1.97 |
2.20 |
nd |
0.87 |
| IP-10 secretion (pg/ml) |
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| None |
82 ± 10 |
170 ± 40 |
nd |
157 ± 34 |
| hTWEAK(10 ng/ml) |
2032 ± 321 |
217 ± 75 |
nd |
3274 ± 69 |
| TNF(1 ng/ml) |
331 ± 57 |
253 ± 73 |
nd |
78 ± 42 |
|
Values for IL-8, matrix metalloproteinase-1 (MMP-1), prostaglandin E2 (PGE-2) and interferon-γ-inducible protein-10 (IP-10) are shown as mean ± SD; similar results were obtained in 3 independent experiments. Single analyses for RANTES (regulated on activation, normal T expressed and secreted) were done because the amount of available medium was limited. hTWEAK, human TNF-like weak inducer of apoptosis; nd, not done; TNF, tumour necrosis factor. | ||||
Chicheportiche et al. Arthritis Res 2002 4:126 doi:10.1186/ar388 |
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