|
Effects of anti-TWEAK monoclonal antibodies on the secretion of proinflammatory molecules by dermal fibroblasts stimulated by hTWEAK, alone or in association with TNF or IL-1β |
||||||||
| BCB10 |
BEB3 |
Stimulation |
BCB10 |
BEB3 |
Stimulation |
|||
| Stimulus |
None |
(10 μg/ml) |
(10 μg/ml) |
index* |
None |
(10 μg/ml) |
(10 μg/ml) |
index* |
|
|
||||||||
| IL-8 secretion (ng/ml) |
IL-6 secretion (ng/ml) |
|||||||
| Medium alone |
6.4 |
8.3 |
5.4 |
1.0 |
1.1 |
1.4 |
0.9 |
1.0 |
| hTWEAK (50 ng/ml) |
55.1 |
9.6 |
96.0 |
17.8 |
6.0 |
2.2 |
15.9 |
17.7 |
| TNF (1 ng/ml) |
111.6 |
126.1 |
114.3 |
1.0 |
11.2 |
11.4 |
10.8 |
1.0 |
| TNF + hTWEAK |
264.3 |
136.7 |
446.1 |
3.9 |
21.1 |
13.9 |
42.9 |
4.0 |
| IL-1β (0.1 ng/ml) |
214.9 |
248.6 |
190.2 |
1.0 |
42.5 |
60.9 |
53.5 |
1.0 |
| IL-1β + hTWEAK |
439.7 |
277.2 |
583.1 |
3.1 |
69.8 |
59.3 |
116.8 |
2.2 |
| MMP-1 secretion (μg/ml) |
IP-10 secretion (pg/ml) |
|||||||
| Medium alone |
0.44 |
0.53 |
0.38 |
1.0 |
18.5 |
nd |
nd |
1.0 |
| hTWEAK (50 ng/ml) |
1.68 |
0.82 |
2.46 |
6.5 |
388 |
nd |
625 |
33.8 |
| TNF (1 ng/ml) |
2.36 |
2.52 |
2.49 |
1.0 |
241 |
85 |
93 |
1.0 |
| TNF + hTWEAK |
2.38 |
3.09 |
3.20 |
1.3 |
6358 |
600 |
6403 |
68.8 |
| IL-1β (0.1 ng/ml) |
2.48 |
2.82 |
2.05 |
1.0 |
175 |
103 |
111 |
1.0 |
| IL-1β + hTWEAK |
3.8 |
2.89 |
3.59 |
1.8 |
272 |
83 |
407 |
3.7 |
| PGE2 secretion (ng/ml) |
RANTES secretion (ng/ml) |
|||||||
| Medium alone |
15 |
11 |
10 |
1.0 |
0.79 |
0.63 |
0.55 |
1.0 |
| hTWEAK (50 ng/ml) |
22 |
8 |
31 |
3.1 |
10.56 |
0.77 |
15.72 |
28.6 |
| TNF (1 ng/ml) |
71 |
41 |
57 |
1.0 |
2.04 |
2.65 |
1.99 |
1.0 |
| TNF + hTWEAK |
125 |
104 |
177 |
3.1 |
77.88 |
5.27 |
91.96 |
46.2 |
| IL-1β (0.1 ng/ml) |
139 |
156 |
259 |
1.0 |
1.16 |
1.30 |
1.49 |
1.0 |
| IL-1β + hTWEAK |
143 |
186 |
312 |
1.2 |
29.28 |
1.57 |
51.78 |
34.7 |
|
* Stimulationindices were calculated from the values obtained with the BEB3 anti-TWEAK mAb results: TWEAK/medium; (TNF + TWEAK)/TNF alone; (IL-1β + TWEAK)/IL-1β alone. Similar results were obtained in 3 independent experiments. hTWEAK, human TNF-like weak inducer of apoptosis; IP-10, interferon-γ-inducible protein-10; MMP-1, matrix metalloproteinase-1; nd, not detectable; PGE2, prostaglandin E2; RANTES, regulated on activation, normal T expressed and secreted; TNF, tumour necrosis factor. | ||||||||
Chicheportiche et al. Arthritis Res 2002 4:126 doi:10.1186/ar388 |
||||||||