Table 1b |
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|
b) U.V. study : The absorption spectra of : (i) diluted serum. (ii) just of the addition of the 0.4 mol/L sod. hydroxide solution. (iii) After 30 minutes incubation with the 0.4 mol/L sodium hydroxide solution. |
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| W.L |
Absorbance |
||
|
|
|||
| (i) |
(ii) |
(iii) |
|
|
|
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| 270 |
0.32 |
0.500 |
0.55 |
| 275 |
0.36 |
0.470 |
0.58 |
| 280 |
0.42 |
0.440 |
0.68* |
| 283 |
0.43* |
0.520 |
0.67 |
| 285 |
0.42 |
0.520 |
0.65 |
| 290 |
0.34 |
0.510 |
0.55 |
| 293 |
0.30 |
0.525* |
0.52 |
| 295 |
0.25 |
0.520 |
0.52 |
| 300 |
0.17 |
0.515 |
0.52 |
|
|
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|
(i) Shows max. absorbance at 283 nm. (ii) Shows max. absorbance at 293 nm. (iii) Shows max. absorbance at 280 nm. |
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|
Nabil Hegazi Arthritis Res 2001 3:E004 doi:10.1186/ar314 |
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