RT-PCR analysis for expression of mRNA for (a) the chemokine SDF-1 and (b) the housekeeping gene GA3PD in cultured BMPCs (GA42, GA43), and a rheumatoid arthritis synovial fibroblast line (RA505 passage 4). Note the similar RT-PCR fragment size in the samples to that in an SDF-1 plasmid (lane 5) included as a positive control. Conditions for RT-PCR and the primers used are described in the methods section.
Zvaifler et al. Arthritis Res 2000 2:477-488 doi:10.1186/ar130