Appearance of cells isolated from a BMPC-rich elutriation fraction of healthy human blood and cultured in DMEM-20% FCS for 8 days (a, b). At this time, the predominant cells consist of both fibroblast-like cells with a central nucleus, filmy cytoplasm, and adherent pseudopods, and large, round cells with a thin, adherent cytoplasm and a round, central nucleus. Hematoxylin staining. (a) ×200; (b) ×400. (c) The appearance of cells isolated from the same BMPC-rich elutriation fraction of healthy human blood and cultured in DMEM-20% FCS supplemented at the initiation of culture with dexamethasone (10-7mol), ascorbic-acid-2-phosphate (0.05 mmol), and β-glycerophosphate (10 mmol) for 8 days. At this time, all the cells have a round or cuboidal morphology with a centrally placed nucleus. ×200. Compare with the nonsupplemented cultures in (a).
Zvaifler et al. Arthritis Res 2000 2:477 doi:10.1186/ar130