Figure 4.
Fluorescence-based X-inactivation using PCR-based AR methylation assay. (a) An example of equivalent inactivation (51% versus 49%) of both upper (271 bp) and
lower (260 bp) alleles. (b) An example of extremely skewed inactivation (10% versus 90%) of the upper (284 bp)
and lower (271 bp) alleles. Mock-digested DNA (without HpaII) was used as control
for peak intensities of both alleles.
Chitnis et al. Arthritis Res 2000 2:399 doi:10.1186/ar118