Table 3

Interactions between extracellular matrix (ECM) proteins and fibroblast-like synoviocytes (FLS)

ECM

Effect

Reference


Laminin (Ln)

Ln is synthesized in rat and human FLS, and is involved in FLS adhesion

[46]

FLS adhesion to Ln shows enhanced proliferative ability in response to PDGF

[47]

RA-FLS bind to Ln more strongly than normal FLS, with monoclonal antibodies to integrin α 3, α 6,

[48]

β 1 subunits partly blocking this adhesion

Fibronectin (Fn)

FLS plated on the substrate containing Fn show extensive focus formation, and enhanced adhesion

[49]

and proliferation

CS-1 Fn correlates with FLS proliferation

[50]

ED-A Fn is associated with activation of FLS

[44]

FLS adhering to Fn show higher proliferative ability in response to PDGF

[43]

Adhesion to Fn through integrin α 5β 1 downregulates the collagenase expression in human FLS

[47]

RA-FLS bind more strongly to Fn than normal FLS; anti-α 5, or β 1 monoclonal antibodies block the

[48]

adhesion

Rabbit FLS cultured on the substrate containing Fn fragment show upregulated expression of

[51]

procollagenase and prostromelysin

Vitronectin (Vn)

FLS adhering to Vn shows higher proliferative ability in response to PDGF. Adhesion to Vn through

[47]

integrin α v downregulates collagenase expression in human FLS

Tenascin (Tn)

FLS synthesize Tn

[52]

RA-FLS bind more strongly to Tn than normal FLS; monoclonal antibodies to integrin β 1 block adhesion

[48]

Rabbit FLS cultured on Tn/Fn mixed substrate show increased expression of collagenase, stromelysin,

[53]

the 92 kDa gelatinase, and c-fos

Hyaluronan (HA)

Synthesized by FLS, degraded by macrophage-like lining cells

[54]

HA inhibits proliferation of FLS

[55]

Decorin

Modulates MMP-1 gene expression of rabbit FLS when present on the substrate with Vn or Fn fragment

[56]

Perlecan

Involved in adhesion and growth of FLS

[57]

Collagen type I

FLS adhering to collagen type I show higher proliferative ability in response to PDGF. Adhesion to

[47]

collagen type I through integrin β 1 downregulates the collagenase expression in human FLS

Collagen type IV

Synthesized by FLS

[58]

RA-FLS bind more strongly to collagen type IV than normal FLS

[48]

monoclonal antibodies to integrin β 1 block adhesion

[59]

Degraded by MMP-2, MT-MMP

[60]

Degraded by matrilysin

[61]


CS-1, connecting sequence 1; ED-A, extra domain-A; MMP, matrix metalloproteinase; MT, membrane type; PDGF, platelet derived growth factor; RA, rheumatoid arthritis.

Konttinen et al. Arthritis Res 2000 2:348-355   doi:10.1186/ar111