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Review

What you should know about PR3-ANCA: Structural aspects of antibodies to proteinase 3 (PR3)

Elisabeth Peen1 and Williams Ralph C2

Author Affiliations

1 University of Bergen, Haukeland Hospital, Bergen, Norway

2 University of New Mexico School of Medicine, Albuquerque, New Mexico, USA

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Arthritis Res 2000, 2:255-259  doi:10.1186/ar97

Published: 12 June 2000

Abstract

Reactive antigenic epitopes on presumed autoantigens of biologic interest have been examined by many researchers. The central third complementarity-determining region (CDR3) residues of a human monoclonal anti-proteinase 3 (PR3) antibody contained many negatively charged aspartic acid residues, perhaps contributing to its reactivity with positively charged PR3 regions. Examination of four other human monoclonal anti-PR3 antibodies shows a number of negatively charged residues within their CDR3 regions. Mapping of segments of linear PR3-epitopes reacting with anti-neutrophil cytoplasmic antibodies (ANCA) demonstrated a preliminary estimate of structures contributing to antigenic determinants. T-cell epitopes on PR3 are reported in studies of chronic myeloid leukemia. These T-cell epitopes appear to be human leukocyte antigen (HLA) A2.1 restricted.

Keywords:
anti-neutrophil cytoplasmic antibodies; proteinase 3; three dimensional structure of proteinase 3; anti-proteinase 3 antibodies; Wegener's granulomatosis