Table 1

Real-time PCR determines the gene expression of cytokines in WT FLS andASIC3-/-FLS after incubation in pH 6.0 or 7.4 with or without IL-1β
Cytokine Il-1b treatment pH 7.4 pH 6.0
WT ASIC3-/- WT ASIC3-/-
IL-6 (-) 0.0018 ± 0.0009 0.0007 ± 0.0002 0.0037 ± 0.0028 0.001 ± 0.0004
(+) 0.0886 ± 0.0218 0.1136 ± 0.013 0.1488 ± 0.0503 0.1829 ± 0.0411
MMP-3 (-) 0.0307 ± 0.0042 0.0180 ± 0.0060 0.0373 ± 0.0072 0.0207 ± 0.0097
(+) 1.7975 ± 0.7027 1.7750 ± 0.712 1.81 ± 0.7002 1.8055 ± 0.6042
MMP-13 (-) 0.0642 ± 0.0062 0.0677 ± 0.0308 0.0761 ± 0.006 0.0879 ± 0.0526
(+) 1.5977 ± 0.4506 1.5066 ± 0.5933 1.8362 ± 0.5362 1.5015 ± 0.5819

mRNA expression of IL-6, metalloproteinase (MMP)-3 and MMP-13 all significantly increased in wild-type (WT) fibroblast-like synoviocytes (FLS) and acid-sensing ion channel (ASIC3)-/- FLS treated with IL-1β when compared to FLS without IL-1β. There were no differences between WT FLS treated with pH 6.0 and those treated pH 7.4, and there was no difference between WT FLS and ASIC3-/- FLS. Results are presented as mean ± standard error of the mean.

Gong et al.

Gong et al. Arthritis Research & Therapy 2014 16:R121   doi:10.1186/ar4577

Open Data