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Open Access Research article

Articular cartilage chondrocytes express aromatase and use enzymes involved in estrogen metabolism

Martin Schicht1, Jana Ernst2, Andrea Nielitz2, Lars Fester3, Michael Tsokos4, Saskia S Guddat4, Lars Bräuer1, Judith Bechmann1, Karl-Stefan Delank5, David Wohlrab5, Friedrich Paulsen1 and Horst Claassen2*

Author Affiliations

1 Institute of Anatomy, Department II, Friedrich Alexander University Erlangen-Nϋrnberg, Universitätsstraße 19, 91052 Erlangen, Germany

2 Institute of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Große Steinstraße 52, 06097 Halle (Saale), Germany

3 Institute of Neuroanatomy, University Medical Center Hamburg-Eppendorf Martinistraße 52, 20246 Hamburg, Germany

4 Institute of Legal Medicine and Forensic Sciences, Charité-University Medicine Berlin, Tumstr. 21, 10559 Berlin, Germany

5 University Hospital of Orthopedics and Physical Medicine, Martin Luther University Halle-Wittenberg, Ernst-Grube-Straße 40, 06120 Halle (Saale), Germany

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Arthritis Research & Therapy 2014, 16:R93  doi:10.1186/ar4539

Published: 11 April 2014

Abstract

Introduction

Sex hormones, especially estrogens, have been implicated in articular cartilage metabolism and the pathogenesis of postmenopausal osteoarthritis. The conversion by aromatase (CYP19A1) of androstenedione into estrone (E1) and of testosterone into 17β-estradiol (E2) plays a key role in the endogenous synthesis of estrogens in tissue.

Methods

We analyzed the expression of aromatase (CYP19A1) in immortalized C-28/I2 and T/C-28a2 chondrocytes, as well as in cultured primary human articular chondrocytes and human articular cartilage tissue, by means of RT-PCR, Western blotting and immunohistochemistry. By means of quantitative RT-PCR and enzyme-linked immunosorbent assay, we also determined whether the aromatase inhibitor letrozole influences estrogen metabolism of cultured chondrocytes in immortalized C-28/I2 chondrocytes.

Results

Aromatase mRNA was detected in both immortalized chondrocyte cell lines, in cultured primary human chondrocytes, and in human articular cartilage tissue. By means of Western blot analysis, aromatase was detected at the protein level in articular cartilage taken from various patients of both sexes and different ages. Cultured primary human articular chondrocytes, C-28/I2 and T/C-28a2, and human articular cartilage tissue reacted with antibodies for aromatase. Incubation of C-28/I2 chondrocytes with 10−11 M to 10−7 M letrozole as an aromatase inhibitor revealed significantly increased amounts of the mRNAs of the enzyme cytochrome P4501A1 (CYP1A1), which is involved in the catagen estrogen metabolism, and of the estrogen receptors ER-α and ER-β. Concomitantly, synthesis of estrone (E1) was significantly downregulated after incubation with letrozole.

Conclusions

We demonstrate that human articular cartilage expresses aromatase at the mRNA and protein levels. Blocking of estrone synthesis by the aromatase inhibitor letrozole is counteracted by an increase in ER-α and ER-β. In addition, CYP1A1, an enzyme involved in catabolic estrogen metabolism, is upregulated. This suggests that articular chondrocytes use ERs functionally. The role of endogenous synthesized estrogens in articular cartilage health remains to be elucidated.