Additional file 1: Figure S1.

Mer and Tyro3 expression in cell lines. (A) Cell lines U937, phorbol myristate acetate-stimulated THP-1, and Jurkat stained with monoclonal anti-Mer-PE. (B) K562 cells stained with monoclonal anti-Tyro3-PE Figure S2. Comparison of Mer and Tyro3 expression in leukocyte populations in the blood of normal healthy subjects and SLE patients. (A) Expression of Mer on lymphocyte populations in normal individuals and patients with SLE. (B) Expression of Tyro3 on leukocyte subpopulations from normal individuals and patients with SLE. Figure S3. Comparison of Mer expression in normal females with SLE patients and effect of gender on expression of Mer. (A) Mer expression levels on monocytes from normal females and patients with SLE. (B) Mer-expression levels on dendritic cells from normal females and patients with SLE. (C) Mer expression levels on monocytes from normal female and male subjects. (D) Mer-expression levels on dendritic cells from normal female and male subjects. Figure S4. Comparison of monocyte and dendritic cells proportions in normal healthy subjects and SLE patients. (A) Monocyte population proportions of peripheral blood mononuclear cells are similar between SLE and normal healthy subjects. The bars represent the mean values. (B) CD1c+ and plasmacytoid dendritic cell proportions of peripheral blood mononuclear cells are reduced in patients with SLE compared with normal control subjects. Figure S5. Mer expression on monocytes, sMer in blood, and proportions of monocyte subsets correlate with IFN-I activity in SLE patients that do not receive prednisone. Mer levels on monocyte subsets. (A) CD14++CD16+, (B) CD14intCD16+, and (C) CD14++CD16- and sMer levels in (D) plasma, positively correlate with interferon activity. Proportions of monocyte subsets correlate with IFN-I activity, negatively for (E) CD14intCD16+ monocytes, and positively for (F) CD14++CD16- monocytes.

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Hilliard et al. Arthritis Research & Therapy 2014 16:R76   doi:10.1186/ar4517