Table 2

Mass spectrometry and DIGE quantification data of synovial membrane proteins
Spot number Protein Gene symbol Accession # Mowse score % Sequence coverage CalculatedpI value Nominal mass (Mr) Number matched peptides Normalised volume (Oligo) Normalised volume (Poly) ANOVAP-value
1 No ID - - - - - - - 0.73 3.02 0.001
2 Protein kinase C inhibitor protein 1 KCIP-1 gi|114629705 88 40 4.78 24,166 14 1.07 2.1 0.03
Annexin A5 ANXA5 gi|149698420 79 33 4.98 35,977 10 - - -
3 Myosin regulatory l ight chain 9 MYL9 gi|29568111 52 29 4.8 19,814 8 0.92 2.02 0.04
4 No ID - - - - - - - 0.79 2.35 0.006
5 Beta Actin ACTB gi|15277503 76 21 5.55 40,194 9 1.14 2.28 0.04
6 Beta Actin ACTB gi|15277503 176 37 5.55 40,194 17 0.35 1.24 0.02
7 Fibrinogen gamma chain FGG gi|930064 94 50 6.49 24,109 11 0.88 2.23 0.004
8 Beta actin variant ACTB gi|62897625 451 53 5.37 41,738 23 0.56 1.94 0.005
9 No ID - - - - - - - 0.64 2.04 0.02
10 Chain A, profi l in-beta-actin PFN1 gi|157111829 106/130 44 5.29 41,616 21 0.39 1.76 0.04
11 F-actin capping protein alpha-1 CAPZA1 gi|5453597 196 55 5.45 32,902 17 0.3 1.38 0.03
12 Myosin regulatory l ight chain 2 MYL2 gi|222144328 86 55 4.26 17,745 10 0.66 2.03 0.02
13 No ID - - - - - - - 0.62 1.72 0.05
14 Peroxiredoxin 2 PRDX2 gi|4758638 348 57 6 25,019 18 0.3 1.73 0.02
15 Chain C, fibrinogen fragment D FFD gi|2781209 89 42 5.87 36,157 12 2.08 0.58 0.0002
16 Fibrinogen gamma chain FGG gi|930064 71 44 6.49 24,109 7 0.9 2.14 0.05
17 Collagen, type VI, alpha 2 COL6A2 gi|41350923 81 18 5.85 182,860 18 2.77 1.09 0.03
18 Chain C, fibrinogen fragment D FFD gi|24987625 102 44 5.86 35,155 14 2.1 0.96 0.005
19 No ID - - - - - - - 1.6 0.9 0.008
20 No ID - - - - - - - 1.6 0.8 0.02
21 No ID - - - - - - - 1.4 0.7 0.006
22 No ID - - - - - - -
23 Integrin, alpha 2b ITGA2B gi|119571979 95 15 5.41 103,127 16 1.87 0.64 0.04
24 Alpha 3 type VI collagen COL6A3 gi|62088852 119 17 8.73 182,860 27 1.78 0.78 0.05
25 No ID - - - - - - - 1.72 0.74 0.04
26 No ID - - - - - - - 1.56 0.72 0.02
27 Peroxiredoxin 6 PRDX6 gi|4758638 348 57 6 25,019 18 - - -
28 Triosephosphate isomerase 1 TPI1 gi|17389815 111 53 6.45 26,625 15 - - -
29 Albumin, isoform CRA_j ALB gi|119626073 59 23 6.4 26,233 7 - - -
30 Calreticul in precursor variant CRT gi|62897681 236 29 4.3 46,890 19 - - -
31 Fibrinogen, beta chain FGB gi|70906435 93 34 8.54 55,892 21 - - -

Spot trypsin digests were identified using matrix assisted laser desorption ionization (MALDI-TOF/TOF), correlated to compiled peptide data (Matrix Science). P-values highlight inter-subgroup comparisons which reached statistical significance (P <0.05) by ANOVA. Full mass spectra peak lists for each identified protein are provided in Additional file 1: Table S1.

Finnegan et al.

Finnegan et al. Arthritis Research & Therapy 2014 16:R8   doi:10.1186/ar4434

Open Data