Open Access Highly Accessed Research article

Intra-articular injection of micronized dehydrated human amnion/chorion membrane attenuates osteoarthritis development

Nick J Willett12, Tanushree Thote23, Angela SP Lin12, Shamus Moran3, Yazdan Raji3, Sanjay Sridaran3, Hazel Y Stevens1 and Robert E Guldberg12*

Author Affiliations

1 George W. Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA 30332-0405, USA

2 Parker H. Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA 30332-0405, USA

3 Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 315 Ferst Drive, Atlanta, GA 30332-0405, USA

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Arthritis Research & Therapy 2014, 16:R47  doi:10.1186/ar4476

Published: 6 February 2014

Additional files

Additional file 1: Figure S1:

Gait analysis data. A) Duty cycle. B) Print area. Baseline = naïve rats prior to surgery. No differences in duty cycle or print area were observed in MMT rats compared to baseline. n = 8.

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Additional file 2: Table S1:

Cytokine ELISA data with standard deviation - indicates cytokine content lower than limit of detection.

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Additional file 3: Figure S2:

A-C) 4X Mosaic representative histology images (H & E) of μ-dHACM treated MMT joints. No lesions are observed on the surface. D-F) Zoomed in 10X images show cartilage surface. Only one sample showed surface erosions. G-I) 4X Mosaic representative histology images (H & E) of saline treated MMT joints. Lesion on sample is indicated by green arrow (I). J-L) Zoomed in 10X images show cartilage surface. Saline treated samples display qualitatively greater surface degeneration. (Black boxes indicated 10X zoomed in areas).

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