Additional file 4: Figure S2.

Depletion of soluble CD18 (sCD18) by binding to intercellular adhesion molecule 1 (ICAM-1) expressed on the human umbelical vein cell line EA.hy926 or spondyloarthritis (SpA) fibroblast-like synoviocytes (FLSs). Culture medium supplemented with 50% (vol/vol) normal human serum (NHS) or 50% (vol/vol) synovial fluid mononuclear cell (SFMC) supernatant as sCD18 source were incubated with EA.hy926 or SPA FLS cells, each cell type either cultured in the supplemented media with 10 ng/mL tumor necrosis factor-alpha (TNFα) (to induce ICAM-1 expression) or in the supplemented media without further additions (“Medium”).

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Kragstrup et al. Arthritis Research & Therapy 2014 16:R42   doi:10.1186/ar4471