Additional file 3: Figure S3.

ERK activation is involved in the IL-17-mediated fibroblast proliferation and collagen production. (A) Fibroblast was stimulated with indicated doses of IL-17 in the presence or absence of IL-17 neutralizing antibody for 24 hours; collagen-1 and collagen-3 protein expressions in fibroblast were analyzed with Western blot. (B) Fibroblast was stimulated with IL-17 and different doses of ERK- specific phosphorylation inhibitor-PD98059 for 24 hours; collagen-1 and collagen-3 protein expressions in fibroblast were analyzed with Western blot. (C) Fibroblast was stimulated with IL-17 and ERK specific phosphorylation inhibitor-PD98059 for indicated days, the proliferation of fibroblast was examined by cell counting kit-8.

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Yang et al. Arthritis Research & Therapy 2014 16:R4   doi:10.1186/ar4430