Dose–response effect of visfatin/Nampt on the mRNA expression of interleukin-6 (IL-6), keratinocyte chemoattractant (Kc) and monocyte chemoattractant protein 1 (MCP-1) by murine chondrocytes (from left to right). Murine chondrocytes were treated with recombinant visfatin/nicotinamide phosphoribosyltransferase (Nampt) at 20, 50 and 100 nM for 24 hours. Quantitative reverse transcriptase-polymerase chain reaction analysis of mRNA levels relative to that of murine hypoxanthine–guanine phosphoribosyltransferase. Data are mean ± standard error of the mean of three experiments.
Laiguillon et al. Arthritis Research & Therapy 2014 16:R38 doi:10.1186/ar4467