Figure 5.

Fucosyltransferase 1 (fut1) mediates adhesion of THP-1 cells to rheumatoid arthritis (RA) synovial fibroblasts and mediates their proliferation. The percent adhesion was defined as the number of adherent cells on synovial tissue (ST) sections divided by the number of adherent cells on control sections. (A) Adhesion of THP-1 cells to TNF-α-stimulated fut1 siRNA-transfected RA synovial fibroblasts (62 ± 8% of maximal adhesion) was significantly decreased compared with adhesion of THP-1 cells to TNF-α-stimulated control siRNA-transfected (93 ± 8% of maximal adhesion) or nontreated RA synovial fibroblasts. (B) Intercellular adhesion molecule 1 (ICAM-1) expression on TNF-α-stimulated fut1 siRNA-transfected RA synovial fibroblasts was significantly decreased compared to TNF-α-stimulated control siRNA or nontreated RA synovial fibroblasts. (C) Vascular cell adhesion molecule 1 (VCAM-1) expression on TNF-α-stimulated fut1 siRNA-transfected RA synovial fibroblasts was significantly decreased compared to TNF-α-stimulated control siRNA or nontreated RA synovial fibroblasts. (D) Fut1 siRNA-transfected lipolysaccharide (LPS)-stimulated RA synovial fibroblasts showed reduced proliferation at 4 and 24 hours compared to LPS-stimulated control siRNA-transfected or nontreated RA synovial fibroblasts. The results are shown as fold change in optical density of fut1- or control siRNA-transfected RA synovial fibroblasts normalized to nontreated RA synovial fibroblasts. Means are presented with standard error. *P <0.05 was significant. (n = number of RA patient synovial fibroblast cultures).

Isozaki et al. Arthritis Research & Therapy 2014 16:R28   doi:10.1186/ar4456
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