Open Access Research article

Carprofen inhibits the release of matrix metalloproteinases 1, 3, and 13 in the secretome of an explant model of articular cartilage stimulated with interleukin 1β

Adam Williams1, Julia R Smith2, David Allaway3, Pat Harris3, Susan Liddell4 and Ali Mobasheri1101156789*

Author Affiliations

1 Musculoskeletal Research Group, School of Veterinary Medicine and Science, Faculty of Medicine and Health Sciences, The University of Nottingham, Sutton Bonington Campus, Sutton Bonington LE12 5RD, United Kingdom

2 Bruker UK Limited, Coventry CV4 9GH, United Kingdom

3 WALTHAM® Centre for Pet Nutrition, Waltham-on-the-Wolds, Melton Mowbray, Leicestershire LE14 4RT, United Kingdom

4 Proteomics Laboratory, School of Biosciences, The University of Nottingham, Sutton Bonington Campus, Leicestershire LE12 5RD, United Kingdom

5 The D-BOARD European Consortium for Biomarker Discovery, The University of Nottingham, University Park, Nottingham NG7 2RD, UK

6 Arthritis Research UK Centre for Sport, Exercise and Osteoarthritis, Nottingham University Hospitals, Nottingham NG7 2UH, United Kingdom

7 Arthritis Research UK Pain Centre, The University of Nottingham, Queen’s Medical Centre, Nottingham NG7 2UH, United Kingdom

8 Medical Research Council and Arthritis Research UK Centre for Musculoskeletal Ageing Research, The University of Nottingham, Queen’s Medical Centre, Nottingham NG7 2UH, United Kingdom

9 Center of Excellence in Genomic Medicine Research (CEGMR), King Fahd Medical Research Center (KFMRC), King AbdulAziz University, Jeddah 21589, Kingdom of Saudi Arabia

10 Schools of Life Sciences and Pharmacy, University of Bradford, Richmond Road, Bradford BD7 1DP, United Kingdom

11 Faculty of Medicine and Health Sciences, The University of Nottingham, Sutton Bonington Campus, Sutton Bonington LE12 5RD, United Kingdom

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Arthritis Research & Therapy 2013, 15:R223  doi:10.1186/ar4424

Published: 30 December 2013

Additional files

Additional file 1: Figure S1:

TSP-1 release stimulated by IL-1β is not significantly decreased after carprofen + IL-1β treatment. Western blots for TSP-1 produced a band at about 125 kDa that was detected only in the presence of IL-1β. Cartilage explants were cultured for 6 days with three treatment replicates from the same individual animal (n = 3). Explant treatments: untreated control, IL-1β (10 ng/ml), carprofen (100 μg/ml), or carprofen (100 μg/ml) + IL-1β (10 ng/ml).

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Additional file 2: Figure S2:

Cartilage explant cultures treated with IL-1β or carprofen + IL-1β did not show significant differences in β-actin release. (A) Representative western blot confirming the absence of β-actin in explant cultures that were untreated or treated with carprofen alone. IL-1β and carprofen + IL-1β treatments induced β-actin release, as evidenced by detection of an approximate 42 kDa band. (B) Graphic representation of the densitometric analysis of β-actin bands. An unpaired t-test was applied to assess statistical significance. ns, no significant difference; +, positive control for β-actin (equine chondrocyte lysate). Cartilage explants were cultured for 6 days with three treatment replicates from two animals (n = 6). Explant treatments: untreated control, IL-1β (10 ng/ml), carprofen (100 μg/ml), or carprofen (100 μg/ml) + IL-1β (10 ng/ml).

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