Double immunofluorescence staining of TACE+ cells in rheumatoid arthritis synovial tissue. (A) TACE+ single cells (red) and other cellular markers (green) can be seen. Tumor necrosis factor-converting enzyme (TACE) was colocalized (yellow, arrows) with CD68+ and CD163+ macrophages, CD55+ fibroblast-like synoviocytes, CD19+ B cells and CD31+ endothelial cells. Figures are representative of five rheumatoid arthritis (RA) patients. (B) Gene expression analysis of TACE mRNA by quantitative polymerase chain reaction (qPCR) in RA (n = 22) and gout (n = 12) synovial tissue analyzed by qPCR. Results presented as mean ± standard error of the mean mRNA expression of TACE relative to GAPDH. No significant differences were observed.
Ramos et al. Arthritis Research & Therapy 2013 15:R209 doi:10.1186/ar4403