Open Access Research article

FMS-related tyrosine kinase 3 ligand (Flt3L)/CD135 axis in rheumatoid arthritis

Maria I Ramos12, Samuel Garcia Perez12, Saida Aarrass12, Boy Helder12, Pleun Broekstra12, Daan M Gerlag1, Kris A Reedquist12, Paul Peter Tak134 and Maria C Lebre12*

Author Affiliations

1 Department of Clinical Immunology and Rheumatology, Academic Medical Center/University of Amsterdam, K0-126 Meibergdreef 9, 1105 AZ, Amsterdam, the Netherlands

2 Department of Experimental Immunology, Academic Medical Center/University of Amsterdam, K0-126 Meibergdreef 9, 1105 AZ, Amsterdam, the Netherlands

3 University of Cambrigde, U.K. Trinity Lane, Cambridge CB2 1TN, UK

4 ImmunoInflammation Therapy Area Unit, GlaxoSmithKline, Stevenage, U.K. Gunnels Wood Road, Stevenage Herts SG1 2NY, UK

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Arthritis Research & Therapy 2013, 15:R209  doi:10.1186/ar4403

Published: 6 December 2013

Additional files

Additional file 1:

Contains a description of the methods from Additional files 2, 3 and 4.

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Additional file 2: Figure S1:

Assessment of global ST inflammation. (A-B) IL-6 and IL-8 gene expression level in STs from RA (n=22) and gout (n=12) patients. Gene expression analysis by qPCR showed that IL-6 and IL-8 expression was similar between RA ST compared to gout ST. Each data point represents a single subject. Results are presented as mean±SEM mRNA expression of IL-6 or IL-8 relative to GAPDH. *p < 0.05, **p < 0.01. (C-D) Immunohistochemical analysis of CD68 and CD163 macrophage markers in RA (n=12), gout (n=11) and HI (n=7) STs. CD68 and CD163 macrophage numbers were increased in RA and gout STs compared to HI STs. No differences were observed between RA and gout STs.

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Additional file 3: Figure S2:

Characterization of Flt3L expression in RA paired PBMC and SFMC and in HI PBMC. Intra- and extra-cellular expression of Flt3L by all cell types is shown in terms of percentage or MFI (cellular marker+ Flt3L+). No differences were observed for the percentage or MFI of extracellular or intracellular Flt3L by CD4+ T cells (A), CD8+ T cells (B) or CD19+ B cells (C) in RA compared to HI. Bars represent the mean (±SEM) of 5-10 RA patients and HI. *p < 0.05, **p < 0.01.

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Additional file 4: Figure S3:

Immunofluorescence staining of TACE+ cells in RA ST. (A) Single stainings for TACE+ (red) and other cellular markers (green) can be seen. (B) Double immunofluorescence staining of TACE+ cells in RA ST with CD3+ T cells and vWF+ blood vessels. TACE did not colocalized with CD3+ T cells or with vWF+ blood vessels. Single immunofluorescence stainings for TACE and vWF are also shown. Figures are representative of five RA patients. Original magnification 250x.

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