Frequencies of SF CD56bright and CD56dim NK cells that secrete IFNγ, TNFα and IL-4 in patients with antibiotic-responsive or antibiotic-refractory arthritis. Synovial fluid (SF) cells from six antibiotic-responsive (Resp) and seven antibiotic-refractory (Ref) patients were stimulated with B. burgdorferi at a multiplicity of infection (MOI) of 25:1 for 24 h. For the last 6 h, GolgiStop was added and the presence of IFNγ, TNFα and IL-4 was measured by intracytoplasmic staining and flow cytometry. Symbols indicate individual subjects and horizontal bars represent the median of each group. Statistical analyses were performed by using the Mann-Whitney test.
Katchar et al. Arthritis Research & Therapy 2013 15:R183 doi:10.1186/ar4373