Table 1

Effect of pharmacologic inhibitors on MSU phagocytosis by OBs
Vacuole number (statistics)*
Control 13.1 ± 3.7 (a)
+ MSU 361 ± 27 (b)
Cytochalasin D + MSU 23.2 ± 10.7 (a)
Colchicine + MSU 321 ± 60 (b)
Control 14.5 ± 3.0 (a)
+ MSU 323 ± 32 (b)
Wortmannin + MSU 157 ± 31 (c)
LY4294002 + MSU 146 ± 27 (c)
Control 16.1 ± 3.2 (a)
+ MSU 281 ± 52 (b)
GF109203X + MSU 126 ± 10 (a)
Gö6976 + MSU 95 ± 10 (a)
SB203580 + MSU 612 ± 101 (c)
PD98069 + MSU 157 ± 39 (a)
Control 16.3 ± 3.2 (a)
+ MSU 193 ± 23 (b)
Piceatannol + MSU 82 ± 20 (a)
PP2 + MSU 231 ± 42 (b)

Confluent OBs were preincubated 15 minutes with 1 μM cytochalasin D or 10 μM colchicine at 37°C and then stimulated with 0.5 mg MSU/106 cells for 24 hours. Confluent OBs were preincubated with PI3K inhibitors: wortmannin 50 nM or LY4294002 10 μM, with PKC inhibitors: GF109203X 5 μM or Gö6976 1 μM, with p38 or ERK1/2 inhibitors: SB203580 10 μM or PD98069 20 μM, or with a Syk inhibitor: piceatannol 20 μM and a Src inhibitor: PP2 10 μM, 15 minutes at 37°C and then stimulated with 0.5 mg MSU/106 cells for 24 hours. Cells were analyzed with microscopy (×200 magnification), and results expressed as cumulative data means of values ± SEM of five pictures for each donor. Statistical analysis was performed on vacuole counts by using the repeated measures ANOVA followed by Tukey multiple-comparison test (n = 4 different donors). *Means without a common letter differ: P < 0.05.

Allaeys et al.

Allaeys et al. Arthritis Research & Therapy 2013 15:R176   doi:10.1186/ar4365

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