Figure 7.

NLRP3 upregulates MSU-induced autophagy. OBs were transfected with AllStars Negative Control siRNA or NLRP3-specific siRNAs for 24 hours and then left unstimulated or stimulated with 0.5 mg MSU for 8 hours. (A) Immunoblot analysis using anti-NLRP3 and anti-β-actin antibodies. Densitometric analyses of NLRP3 expression. Data are expressed as arbitrary densitometric units normalized by β-actin levels; NLRP3 was presented as the ratio of NLRP3 levels of MSU-stimulated cells to NLRP3 levels of vehicle-stimulated cells. Statistical analysis was performed by using the paired two-tailed t test (n = 4 different donors). (B) Immunoblot analysis using anti-LC3 and anti-β-actin antibodies. Data are expressed as arbitrary densitometric units normalized by β-actin levels; LC3-II conversion induced by MSU was the ratio of LC3-II levels of MSU-stimulated cells to LC3-II levels of vehicle-stimulated cells. Results are expressed as mean ± SEM (n = 4 different donors). Statistical analysis was performed by using the paired two-tailed t test; *P < 0.05; **P < 0.001.

Allaeys et al. Arthritis Research & Therapy 2013 15:R176   doi:10.1186/ar4365
Download authors' original image