Effects of MSU on OB viability and proliferation. (A) Confluent OBs were incubated with vehicle (control) or with MSU from 0.3 to 1 mg/106 cells for 24, 48, or 72 hours. Cells were washed with PBS and removed with Accutase. Propidium iodide (PI) exclusion was analyzed with cytofluorometry. (B) Cells were plated 3 days with vehicle or with MSU in 96 wells and then analyzed with the CellTiter 96 AQueous One proliferation assay. Data are represented as a ratio of MSU-stimulated to vehicle-stimulated cells. Results are given as mean ± SEM of values from four (A) or three (B) different donors. Statistical analysis: one-way ANOVA followed by Bonferroni multiple-comparison test: ns, nonsignificant; means without a common letter differ: P < 0.05.
Allaeys et al. Arthritis Research & Therapy 2013 15:R176 doi:10.1186/ar4365