Additional file 6: Figure S5.
IL-29 induces production of TLR2, 3 and 4 in MH7A cells. MH7A cells were stimulated with IL-29 (100 ng/ml) or PGN (2.5 μg/ml), poly I:C (25 μg/ml) or LPS (100 ng/ml) (a positive control for TLR2, 3 and 4, respectively) for 24 h, and then the expression of TLR2, 3 and 4 mRNA (A) or protein (B) was measured by real time PCR or flow cytometric analysis, respectively. (C) Quantitative expression of TLR2, 3 and 4 protein was summarized. The data depicted in (A) and (B) show representative of one out three independent experiments. The error bars represent mean ± SD for triplicate wells. The data present in (C) show the means ± SD for 3 independent experiments. *p < 0.05 versus medium control. **p < 0.01 versus medium control.
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Xu et al. Arthritis Research & Therapy 2013 15:R170 doi:10.1186/ar4357