Table 2

Antibodies used to detect phenotype synovium-derived mesenchymal stem cells (SMSCs) with flow cytometry
Antibody Fluorochrome Concentration (μg/ml) Source
CD14 PE 200 Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA)
CD34 PE 50 Southern Biotech (Birmingham, AL, USA)
CD44 FITC 50 Becton Dickinson (Bedford, MA, USA)
CD45 PE 10 Caltag (Burlingame, CA, USA)
CD90 PE 1 Becton Dickinson (Bedford, MA, USA)
CD105 FITC 10 Becton Dickinson (Bedford, MA, USA)
CD166 FITC 50 AbD Serotec (MorphoSys, UK)
HLA-DR PE 200 Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA)

All antibodies were mouse IgG isotype antibodies obtained from commercial sources.

Zhang et al.

Zhang et al. Arthritis Research & Therapy 2013 15:R169   doi:10.1186/ar4355

Open Data