Additional file 1: Figure S1.
Fibroblasts increase B-cell survival in vitro. B cells alone or cocultured with fibroblasts were seeded in 24-well plates for 3 or 5 days. For transwell experiments, B cells (5 × 105 cells) and HDF (105 cells) were seeded in the upper and lower chambers, respectively. After 3 or 5 days, B-cell viability was determined by FACS analysis; vital B cells were brightly positive when stained with DiOC6 and excluded PI. Data are expressed as the median of duplicate samples of two independent experiments ± interquartile range.
Format: PDF Size: 32KB Download file
This file can be viewed with: Adobe Acrobat Reader
François et al. Arthritis Research & Therapy 2013 15:R168 doi:10.1186/ar4352