Expression of collagen and profibrotic markers is increased in SScHDF cocultured with B cells. (A) Levels of COL1A1, COL1A2, and COL3A1 mRNA were determined with RT-qPCR in SScHDF cocultured with blood B cells (black bars) for 3 days. Results were normalized to GAPDH and were expressed as the fold change compared with samples from SScHDF alone (white bars). (B) Collagen release by SScHDF was determined by the Sircol collagen dye-binding assay in 3- and 5-day culture supernatants. (C) Levels of α-SMA, MMP9, and TIMP1 mRNA were determined with RT-qPCR in the same conditions as panel A. Data are expressed as the median of duplicate samples of six independent experiments ± interquartile range. *P < 0.05; **P < 0.01; ***P < 0.001.
François et al. Arthritis Research & Therapy 2013 15:R168 doi:10.1186/ar4352