Open Access Research article

B lymphocytes and B-cell activating factor promote collagen and profibrotic markers expression by dermal fibroblasts in systemic sclerosis

Antoine François1, Emmanuel Chatelus2, Dominique Wachsmann1, Jean Sibilia12, Seiamak Bahram1, Ghada Alsaleh1 and Jacques-Eric Gottenberg12*

Author Affiliations

1 Immunorhumatologie Moléculaire, INSERM UMR_S 1109, Centre de Recherche en Immunologie et Hématologie, Fédération de Médecine Translationnelle de Strasbourg, Université de Strasbourg, Strasbourg, France

2 Service de Rhumatologie, Centre National de Référence pour les Maladies Systémiques Autoimmunes Rares, Hôpitaux Universitaires de Strasbourg, Avenue Moliere, 67098 Strasbourg Cedex, France

For all author emails, please log on.

Arthritis Research & Therapy 2013, 15:R168  doi:10.1186/ar4352

Published: 28 October 2013

Additional files

Additional file 1: Figure S1:

Fibroblasts increase B-cell survival in vitro. B cells alone or cocultured with fibroblasts were seeded in 24-well plates for 3 or 5 days. For transwell experiments, B cells (5 × 105 cells) and HDF (105 cells) were seeded in the upper and lower chambers, respectively. After 3 or 5 days, B-cell viability was determined by FACS analysis; vital B cells were brightly positive when stained with DiOC6 and excluded PI. Data are expressed as the median of duplicate samples of two independent experiments ± interquartile range.

Format: PDF Size: 32KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data