Th17 clones enhance MCP-1, IL-8 and MMP-1 and decrease type I collagen production by fibroblasts. (A) MCP-1, IL-8, MMP-1 and type I collagen production by HD fibroblasts cultured for 48 hours in the presence of supernatants of a prototypical Th17 clone supernatant before (-) or after (+) CD3/CD28 activation. TNF (1 ng/ml) and TGF-β (10 ng/ml) were used as positive controls. (B) Fold change relative to the control condition (dotted line) of MCP-1, IL-8, MMP-1 and type I collagen production by HD (gray) and SSc (black) fibroblasts cultured for 48 hours in the presence of supernatants from five distinct, activated Th17 clones. Each symbol represents an individual clone and the solid horizontal lines depict the median. Significant differences relative to the control condition were assessed by the Wilcoxon signed-rank test: * = P <0.05. Significant differences between HD and SSc are shown.
CD: cluster of differentiation; HD: healthy donors; IL: interleukin; MCP-1: monocyte chemotactic protein-1; MMP-1: matrix metalloprotein-1; SSc: systemic sclerosis.
Brembilla et al. Arthritis Research & Therapy 2013 15:R151 doi:10.1186/ar4334