IL-17A enchances the mRNA levels of MCP-1, IL-8 and MMP-1 with no effects of COL1A1 and COL1A2 in HD and SSc fibroblasts. (A-B) Steady-state mRNA levels were quantified by real-time PCR after 24 hours of culture. Expression levels were normalized against the geometric mean of two house-keeping genes (GADPH, EEF1A1). In all panels, the results are expressed as fold change relative to the control condition (medium with no IL-17A, dotted line) Each symbol represents a distinct individual and the horizontal lines depict the median. Significant differences relative to the control condition were assessed by the Wilcoxon signed-rank test. Fold increase induced by positive control TNF: 28 ± 38 (CCL2), 448 ± 317 (IL8), 18 ± 22 (MMP1), 1.5 ± 0.5 (MMP2), 1.6 ± 0.7 (TIMP1) and TGF-β: 3.8 ± 2.1 (COL1A1), 1.8 ± 0.6 (COL1A2).
CCL: CC-chemokine ligand; COL: collagen; EEF1A1: eukaryotic elongation factor 1 alpha 1; GADPH: glyceraldehyde 3 phosphate dehydrogenase; HD: healthy donors; IL: interleukin; MCP-1: monocyte chemotactic protein-1; MMP-1: matrix metalloprotein-1; PCR: polymerase chain reaction; SSc: systemic sclerosis; TGF: transforming growth factor; TIMP: tissue inhibitor of matrix metalloproteinase; TNF: tumor necrosis factor.
Brembilla et al. Arthritis Research & Therapy 2013 15:R151 doi:10.1186/ar4334