Additional file 2: Figure S3.
Effect of IFN-α on intracellular cytokine expression in mock, mBSA and anti-CD3-restimulated T cells and mock and mBSA-stimulated macrophages from splenocytes during AIA. Spleens were collected from mice at days 0, 14, 21, and 28 during the course of AIA and restimulated with complete media (mock) or mBSA (50 μg/ml) or anti-CD3 (1 μg/ml) for 24 hours. Brefeldin A (5 μg/ml) and monensin (1 μg/ml) were added 5 hours before FACS analysis. (A-F) show percentage IFN-γ-, IL-17A-, and TGF-β-expressing spleen cells of gated CD4+(A-C) and F4/80+ cells (D-F). The data shown are mean values ± SEM of pooled data from two independent experiments (n ≥ 5) with similar results (**P < 0.01). Blue line, mice immunized in the presence of IFN-α; black line, control.
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Chalise et al. Arthritis Research & Therapy 2013 15:R143 doi:10.1186/ar4326