Additional file 1: Figure S1.
Effect of IFN-α administration on antigenic specific total IgG and IgG subtypes during AIA in absence of adjuvant. Mice were injected twice with mBSA with or without 1,000 U IFN-α in 1-week intervals followed by intraarticular injection of mBSA in PBS 21 days later. Serum was collected at days 0, 14, 21, and 28 and analyzed for mBSA-specific antibodies with ELISA by using detection antibodies specific for total IgG, IgG1, IgG2a, and IgG2b. Black bars represent control group without IFN-α treatment, and blue bars represent IFN-α-treated group. Data are expressed as the mean absorbance (450 nm) ± SEM, n ≥ 6.
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Chalise et al. Arthritis Research & Therapy 2013 15:R143 doi:10.1186/ar4326