IFN-α modulates antigen-induced cytokine production from splenocytes and lymph node cells ex vivo. Spleens and draining lymph nodes were collected from mice at days 0, 14, 21, and 28 during the course of AIA. Single-cell suspensions were prepared and were restimulated with 50 μg/ml of mBSA for 48 hours. The supernatant of the culture was analyzed for different cytokines with Luminex (IL-1β, IL-6, IL-10, IL-12, IL-13, IL-17, IFN-γ, and TNF) and ELISA (TGF-β). The data are expressed as mean pg/ml ± SEM (n ≥ 6) in (A) splenocytes and (B) lymph node cells (n ≥ 6). From each value, the background cytokine levels found in culture media (including fetal bovine serum) were subtracted. The black line and the blue line represent groups receiving 0 U and 1,000 U of IFN-α, respectively, at the time of mBSA immunizations. Mann–Whitney U test was performed to compare cytokine levels between the two groups (*P < 0.05; **P < 0.01).
Chalise et al. Arthritis Research & Therapy 2013 15:R143 doi:10.1186/ar4326