Table 1

Peptide sets immobilized on the microarrays for surface plasmon resonance imaging analysisa
Set Origin Peptide sequence Position of citrulline residuesb
1 A α-enolase (cyclic) CKIHAREIFDSRGNPTVECZO
B CKIHAXEIFDSXGNPTVECZO 9, 15
2 A β-actin MKILTERGYSFTTAEREIVRDIKEKLZO
B MKILTEXGYSFTTAEXEIVXDIKEKLZO 196, 206, 210
3 A CCP2-like (cyclic) c
B n.r.
4 A Collagen type II GLPGVKGHRGYPGLDGAZO
B GLPGVKGHXGYPGLDGAZO 290
5 A Fibrinogen FLAEGGGVRGPRVVERHZO
B FLAEGGGVXGPRVVERHZO α35d
6 A Fibrinogen TSSTSYNRGDSTFZO
B TSSTSYNXGDSTFZO α591d
7 A Fibrinogen NEEGFFSARGHRPLDKKZO
B NEEGFFSAXGHRPLDKKZO β44e
8 A Fibrinogen EEAPSLRPAPPPISGGGYRARPAKAAAZO
B EEAPSLXPAPPPISGGGYXAXPAKAAAZO β60, 72, 74e
9 A Fibronectin LTVGLTRRGQPRQYZO
B LTVGLTXXGQPRQYZO 1,035, 1,036
10 A Fibronectin YNQYSQRYHQRTNZO
B YNQYSQXYHQRTNZO 2356
11 A Filaggrin (cfc1; cyclic) HQCESTRGRSRGRCGRSGSZO f
B HQCESTXGRSRGRCGRSGSZO
12 A MNDA KLTSEARGRIPVAQKZO
B KLTSEAXGXIPVAQKZO 127, 129
13 A Peptide library c
B n.r.
14 A Peptide library c
B n.r.
15 A Peptide library c
B n.r.
16 A Peptide library c
B n.r.
17 A Vimentin MSTRSVSSSSYRRMFGGPZO
B MSTXSVSSSSYXXMFGGPZO 4, 12, 13
18 A Vimentin GVYATRSSAVRLRSSVPGZO
B GVYATXSSAVXLXSSVPGZO 64, 69, 71
19 A Vimentin LTAALRDVRQQYESZO
B LTAALXDVXQQYESZO 270, 273
20 A Vimentin SLNLRETNLDSLPLVDZO
B SLNLXETNLDSLPLVDZO 424

aCCP2, cyclic citrullinated peptide 2; MNDA, myeloid cell nuclear differentiation antigen; n.r., not relevant; O, biotinylated lysine; X, citrulline; Z, aminohexanoic acid. bAmino acid position in the corresponding polypeptide relative to the N-terminal methionine of the primary translation product. cSequence is defined in patent EP2071335. dα-Chain of fibrinogen. eβ-Chain of fibrinogen. fDerived from sequence presented in [27].

van Beers et al.

van Beers et al. Arthritis Research & Therapy 2013 15:R140   doi:10.1186/ar4322

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