Additional file 5.

Standard curves defined with positive control samples. A mixture of standard cytokines was prepared according to the supplier’s protocol (eBioscience) and serially diluted. To determine cytokine concentration, a fluorescence-activated cell sorting analysis was performed on a BD FACS Canto and data evaluated with FlowCytomix Pro 3.0 software (eBioscience), generating the standard curves. Manually a level of quantification (LOQ) was superimposed on these standard curves (dotted green line).

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Doll et al. Arthritis Research & Therapy 2013 15:R138   doi:10.1186/ar4319