Additional file 2.

Complete sequence of F8-muIL10.‚ÄČThe sequence for murine IL10 (amino acids 19 to 178) was appended at the C-terminus of the F8 antibody in diabody format, with a five-amino-acid linker between variable heavy chain (VH) and variable light chain (VL) and a 15-amino-acid linker ((SSSSG)3) between the antibody and IL-10. At the N-terminus a signal sequence (SS) was added. By NheI and NotI double digest the insert was included into the mammalian cell-expression vector pcDNA3.1(+).

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Doll et al. Arthritis Research & Therapy 2013 15:R138   doi:10.1186/ar4319