Additional file 1.
Complete sequence of muTNFR-Fc. The sequence for murine TNFR (amino acids 23 to 258) was directly fused to the murine Fc fragment (amino acids 98 to 324), containing the hinge, CH2 and CH3 regions. At the N-terminus a signal sequence (SS) was added. By HindIII and NotI double digest the insert was included into the mammalian cell-expression vector pcDNA3.1(+).
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Doll et al. Arthritis Research & Therapy 2013 15:R138 doi:10.1186/ar4319