Incubation experiment of radiolabeled immunocytokines with blood, analyzed by a centrifugation-based assay. (a) Different concentrations of F8-huIL10 were incubated with fresh human blood. (b) Different concentrations of F8-muIL10 were incubated with fresh mouse blood. In both cases, inhibitors of coagulation were used. After a centrifugation step and separation of plasma from the cell pellet, radioactivity was counted and expressed as percent of binding.
Doll et al. Arthritis Research & Therapy 2013 15:R138 doi:10.1186/ar4319